研究業績

2024

New！
259) Shionoya K, Park J, Ekimoto T, Takeuchi J, Mifune J, Morita T, Ishimoto N, Umezawa H, Yamamoto K, Kobayashi C, Kusunoki A, Nomura N, Iwata S, Muramatsu M, Tame J.R.H, Ikeguchi M, Park S*, Watashi K*.

“Structural basis for hepatitis B virus restriction by a viral receptor homologue.”

Nat Commun. 15: 9241 (2024)

DOI: DOI: 10.1038/s41467-024-53533-6 PubMed ID: 39455604

New！
258) Miyata Y, Takahashi K, Lee Y, Sultan C.S, Kuribayashi R, Takahashi M, Hata K, Bamba T, Izumi Y, Liu K, Uemura T, Nomura N, Iwata S, Nagata S, Nishizawa T*, Segawa K*.

“Membrane structure-responsive lipid scrambling by TMEM63B to control plasma membrane lipid distribution.”

Nat Struct Mol Biol. (2024)

DOI: DOI: 10.1038/s41594-024-01411-6 PubMed ID: 39424995

257) Im D*, Jormakka M, Juge N, Kishikawa J, Kato T, Sugita Y, Noda T, Uemura T, Shiimura Y, Miyaji T, Asada H, Iwata S*.

“Neurotransmitter recognition by human vesicular monoamine transporter 2.”

Nat Commun. 15(1):7661. (2024)

DOI: DOI: 10.1038/s41467-024-51960-z.PubMed ID: 39284862

256) Oguma T#, Jino K#, Nakahara K#, Asada H#, Fuchino K, Nagatani K, Kouki K, Okamoto R, Takai N, Koda K, Fujita S, Sekiguchi Y, Yasuo K, Mayumi K, Abe A, Imono M, Horiguchi N, Iwata S*, Kusakabe KI*. (#Contributed equally)

“Dual 5-HT2A and 5-HT2C Receptor Inverse Agonist That Affords In Vivo Antipsychotic Efficacy with Minimal hERG Inhibition for the Treatment of Dementia-Related Psychosis.”

J Med Chem. 22;67(16):14478-14492 (2024)

DOI: DOI: 10.1021/acs.jmedchem.4c01244.PubMed ID: 39137033

255) Maity B*, Shoji M*, Luo F, Nakane T, Abe S, Owada S, Kang J, Tono K, Tanaka R, Pham TT, Kojima M, Hishikawa Y, Tanaka J, Tian J, Nagama M, Suzuki T, Noya H, Nakasuji Y, Asanuma A, Yao X, Iwata S, Shigeta Y, Nango E*, Ueno T*.

“Real-time observation of a metal complex-driven reaction intermediate using a porous protein crystal and serial femtosecond crystallography.”

Nat Commun. 15(1):5518. (2024)

DOI: DOI: 10.1038/s41467-024-49814-9.PubMed ID: 38951539

254) Li H, Nakajima Y, Nango E, Owada S, Yamada D, Hashimoto K, Luo F, Tanaka R, Akita F, Kato K, Kang J, Saitoh Y, Kishi S, Yu H, Matsubara N, Fujii H, Sugahara M, Suzuki M, Masuda T, Kimura T, Thao TN, Yonekura S, Yu LJ, Tosha T, Tono K, Joti Y, Hatsui T, Yabashi M, Kubo M, Iwata S, Isobe H, Yamaguchi K, *Suga M. and *Shen JR.

“Oxygen-evolving photosystem II structures during S1-S2-S3 transitions.”

Nature. 626(7999):670-677. (2024)

DOI: DOI: 10.1038/s41586-023-06987-5.PubMed ID: 38297122

253) Asami J#, Park JH#, Nomura Y#, Kobayashi C#, Mifune J, Ishimoto N, Uemura T, Liu K, Sato Y, Zhikuan Z, Muramatsu M, Wakita T, Drew D, Iwata S, Shimizu T, *Watashi K, *Park SY, *Nomura N. and *Ohto U. (#Contributed equally)

“Structural basis of hepatitis B virus receptor binding”

Nat Struct Mol Biol. (2024)

DOI: DOI: 10.1038/s41594-023-01191-5.

252) Imamura K, Akagi KI, Miyanoiri Y, Tsujimoto H, Hirokawa T, Ashida H, Murakami K, Inoue A, Suno R, Ikegami T, Sekiyama N, Iwata S, Kobayashi T. and *Tochio H.

“Interaction modes of human orexin 2 receptor with selective and nonselective antagonists studied by NMR spectroscopy”

Structure. S0969-2126(23)00449-5. (2024)

DOI: DOI: 10.1016/j.str.2023.12.008.PubMed ID: 38194963

2023

251) Araya T, Matsuba Y, Suzuki H, Doura T, Nuemket N, Nango E, Yamamoto M, Im D, *Asada H, *Kiyonaka. S and *Iwata S.

“Crystal structure reveals the binding mode and selectivity of a photoswitchable ligand for the adenosine A2A receptor”

Biochem Biophys Res Commun.695:149393. (2023)

DOI: DOI: 10.1016/j.bbrc.2023.149393.PubMed ID: 38171234

250) Safari C, *Ghosh S, Andersson R, Johannesson J, Bath P, Uwangue O, Dahl P, Zoric D, Sandelin E, Vallejos A, Nango E, Tanaka R, Bosman R, Borjesson P, Dunevall E, Hammarin G, Ortolani G, Panman M, Tanaka T, Yamashita A, Arima T, Sugahara M, Suzuki M, Masuda T, Takeda H, Yamagiwa R, Oda K, Fukuda M, Tosha T, Naitow H, Owada S, Tono K, Nureki O, Iwata S, *Neutze R, *Branden G.

“Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome c oxidase”

Sci Adv.9(49):eadh4179. (2023)

DOI: DOI: 10.1126/sciadv.adh4179.PubMed ID: 38064560

249) *Maestre-Reyna M, Wang PH, Nango E, Hosokawa Y, Saft M, Furrer A, Yang CH, Gusti Ngurah Putu EP, Wu WJ, Emmerich HJ, Caramello N, Franz-Badur S, Yang C, Engilberge S, Wranik M, Glover HL, Weinert T, Wu HY, Lee CC, Huang WC, Huang KF, Chang YK, Liao JH, Weng JH, Gad W, Chang CW, Pang AH, Yang KC, Lin WT, Chang YC, Gashi D, Beale E, Ozerov D, Nass K, Knopp G, Johnson PJM, Cirelli C, Milne C, Bacellar C, Sugahara M, Owada S, Joti Y, Yamashita A, Tanaka R, Tanaka T, Luo F, Tono K, Zarzycka W, Muller P, Alahmad MA, Bezold F, Fuchs V, Gnau P, Kiontke S, Korf L, Reithofer V, Rosner CJ, Seiler EM, Watad M, Werel L, Spadaccini R, Yamamoto J, Iwata S, Zhong D, Standfuss J, Royant A, *Bessho Y, *Essen LO, *Tsai MD.

“Visualizing the DNA repair process by a photolyase at atomic resolution.”

J Science.382(6674):eadd7795. (2023)

DOI: DOI: 10.1126/science.add7795.PubMed ID: 38033054

248) Im D, Kishikawa JI, Shiimura Y, Hisano H, Ito A, Fujita-Fujiharu Y, Sugita Y, Noda T, *Kato T, *Asada H. and *Iwata S.

“Structural insights into the agonists binding and receptor selectivity of human histamine H4 receptor.”

Nat Commun.14(1):6538 (2023)

DOI: DOI: 10.1038/s41467-023-42260-z.PubMed ID: 37863901

247) Birch J, Kwan TOC, Judge PJ, Axford D, Aller P, Butryn A, Reis RI, Bada Juarez JF, Vinals J, Owen RL, Nango E, Tanaka R, Tono K, Joti Y, Tanaka T, Owada S, Sugahara M, Iwata S, Orville AM, *Watts A. and *Moraes I.

“A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography”

J Appl Crystallogr. 56(Pt 5):1361-1370 (2023)

DOI: DOI: 10.1107/S1600576723006428.PubMed ID: 37791355

246) Wolff AM, *Nango E, Young ID, Brewster AS, Kubo M, Nomura T, Sugahara M, Owada S, Barad BA, Ito K, Bhowmick A, Carbajo S, Hino T, Holton JM, Im D, O'Riordan LJ, Tanaka T, Tanaka R, Sierra RG, Yumoto F, Tono K, Iwata S, Sauter NK, Fraser JS. and *Thompson MC.

“Mapping protein dynamics at high spatial resolution with temperature-jump X-ray crystallography”

Nat Chem. Online ahead of print (2023)

DOI: DOI: 10.1038/s41557-023-01329-4.PubMed ID: 37723259

245) Hutchison CDM, Baxter JM, Fitzpatrick A, Dorlhiac G, Fadini A, Perrett S, Maghlaoui K, Lefevre SB, Cordon-Preciado V, Ferreira JL, Chukhutsina VU, Garratt D, Barnard J, Galinis G, Glencross F, Morgan RM, Stockton S, Taylor B, Yuan L, Romei MG, Lin CY, Marangos JP, Schmidt M, Chatrchyan V, Buckup T, Morozov D, Park J, Park S, Eom I, Kim M, Jang D, Choi H, Hyun H, Park G, Nango E, Tanaka R, Owada S, Tono K, DePonte DP, Carbajo S, Seaberg M, Aquila A, Boutet S, Barty A, Iwata S, Boxer SG, Groenhof G. and *van Thor JJ.

“Optical control of ultrafast structural dynamics in a fluorescent protein”

Nat Chem Online ahead of print (2023)

DOI: DOI: 10.1038/s41557-023-01275-1.PubMed ID: 37563326

244) Bui HB, Watanabe S, Nomura N, Liu K, Uemura T, Inoue M, Tsutsumi A, Fujita H, Kinoshita K, Kato Y, Iwata S, Kikkawa M. and *Inaba K.

“Cryo-EM structures of human zinc transporter ZnT7 reveal the mechanism of Zn2+ uptake into the Golgi apparatus”

Nat Commun 14(1):4770

DOI: DOI: 10.1038/s41467-023-40521-5.PubMed ID: 37553324

243) Fadini A, Hutchison CDM, Morozov D, Chang J, Maghlaoui K, Perrett S, Luo F, Kho JCX, Romei MG, Morgan RML, Orr CM, Cordon-Preciado V, Fujiwara T, Nuemket N, Tosha T, Tanaka R, Owada S, Tono K, Iwata S, Boxer SG, Groenhof G, *Nango E and *van Thor JJ.

“Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism”

J Am Chem Soc. 145(29): 15796-15808 (2023)

DOI: DOI: 10.1021/jacs.3c02313.PubMed ID: 37418747

242)Miyagi H, Suzuki M, Yasunaga M, Asada H, Iwata S and *Saito JI.

“Structural insight into an anti-BRIL Fab as a G-protein-coupled receptor crystallization chaperone”

Acta Crystallogr D Struct Biol.79(Pt 5):435-441(2023)

DOI: DOI: 10.1107/S205979832300311X.PubMed ID: 37098908

241) Jaunet-Lahary T, *Shimamura T, Hayashi M, Nomura N, Hirasawa K, Shimizu T, Yamashita M, Tsutsumi N, Suehiro Y, Kojima K, Sudo Y, Tamura T, Iwanari H, Hamakubo T, Iwata S, Okazaki KI, *Hirai T and *Yamashita A.

“Structure and mechanism of oxalate transporter OxlT in an oxalate-degrading bacterium in the gut microbiota”

Nat Commun. 14(1):1730 (2023)

DOI: DOI: 10.1038/s41467-023-36883-5.PubMed ID: 37012268

240) Nakata K, *Kashiwagi T, Kunishima N, Naitow H, Matsuura Y, Miyano H, Mizukoshi T, Tono K, Yabashi M, Nango E and *Iwata S.

“Ambient temperature structure of phosphoketolase from Bifidobacterium longum determined by serial femtosecond X-ray crystallography”

Acta Cryst. D. 79(Pt 4):290-303 (2023)

DOI: DOI: 10.1107/S2059798323001638.PubMed ID: 36974963

239) Gruhl T, Weinert T, Rodrigues M, Milne CJ, Ortolani G, Nass K, Nango E, Sen S, Johnson PJM, Cirelli C, Furrer A, Mous S, Skopintsev P, James D, Dworkowski F, Båth P, Kekilli D, Ozerov D, Tanaka R, Glover H, Bacellar C, Brünle S, Casadei CM, Diethelm AD, Gashi D, Gotthard G, Guixà-Gonzàlez R, Joti Y, Kabanova V, Knopp G, Lesca E, Ma P, Martiel I, Mühle J, Owada S, Pamula F, Sarabi D, Tejero O, Tsai CJ, Varma N, Wach A, Boutet S, Tono K, Nogly P, Deupi X, Iwata S, Neutze R, Standfuss J, *Schertler G and *PanneelsV.

“Ultrafast structural changes direct the first molecular events of vision”

Nature 615: 939-944 (2023)

DOI: DOI: 10.1038/s41586-023-05863-6.

238) Ohno Y, Suzuki M, Asada H, Kanda T, Saki M, Miyagi H, Yasunaga M, Suno C, Iwata S, Saito JI, *Uchida S.

“In vitro pharmacological profile of KW-6356, a novel adenosine A2A receptor antagonist/inverse agonist”

Mol Pharmacol. Online ahead of print. (2023)

DOI: DOI: 10.1124/molpharm.122.000633.PubMed ID: 36894319

2022

237) *Murakawa T, Suzuki M, Fukui K, Masuda T, Sugahara M, Tono K, Tanaka T, Iwata S, Nango E, Yano T, Tanizawa K, *Okajima T.

“Serial femtosecond X-ray crystallography of an anaerobically formed catalytic intermediate of copper amine oxidase”

Acta Cryst. D78(Pt 12):1428-1438. (2022)

DOI: DOI: 10.1107/S2059798322010385.PubMed ID: 36458614

236)*Asada H, Im D, Hotta Y, Yasuda S, Murata T, Suno R and *Iwata S.

“Molecular basis for anti-insomnia drug design from structure of lemborexant-bound orexin 2 receptor”

Structure. 30(12):1582-1589.e4 (2022)

DOI: DOI: 10.1016/j.str.2022.11.001.PubMed ID: 36417909

235) Al-Habsi M#, Chamoto K#, Matsumoto K#, Nomura N#, Zhang B, Sugiura Y, Sonomura K, Maharani A, Nakajima Y, Wu Y, Nomura Y, Menzies R, Tajima M, Kitaoka K, Haku Y, Delghandi S, Yurimoto K, Matsuda F, Iwata S, Ogura T, *Fagarasan S, *Honjo T. (#Contributed equally)

“Spermidine activates mitochondrial trifunctional protein and improves antitumor immunity in mice”

Science 378(6618):eabj3510. (2022)

DOI: DOI: 10.1126/science.abj3510.PubMed ID: 36302005

234) *Suno R, Sugita Y, Morimoto K, Takazaki H, Tsujimoto H, Hirose M, Suno-Ikeda C, Nomura N, Hino T, Inoue A, Iwasaki K, Kato T, Iwata S and *Kobayashi T.

“Structural insights into the G protein selectivity revealed by the human EP3-Gi signaling complex”

Cell Reports 40(11), 111323(2022)

DOI: DOI: 10.1016/j.celrep.2022.111323.

233) Zhang Z, Nomura N, Muramoto Y, Ekimoto T, Uemura T, Liu K, Yui M, Kono N, Aoki J, Ikeguchi M, Noda T, Iwata S, Ohto U, Shimizu T.

“Structure of SARS-CoV-2 membrane protein essential for virus assembly.”

Nat Commun. 13(1):4399 (2022)

DOI: DOI: 10.1038/s41467-022-32019-3.PubMed ID: 35931673

232) Liu X, Liu P, Li H, Xu Z, Jia L, Xia Y, Yu M, Tang W, Zhu X, Chen C, Zhang Y, Nango E, Tanaka R, Luo F, Kato K, Nakajima Y, Kishi S, Yu H, Matsubara N, Owada S, Tono K, Iwata S, *Yu LJ, *Shen JR, *Wang J.

“Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser”

Nat Chem.14(9):1054-1060 (2022)

DOI: DOI: 10.1038/s41557-022-00992-3.PubMed ID: 35851837

231) Asami J# , Terakado-Kimura K# , Fujita-Fujiharu Y# , Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, *Nomura N and *Ohto U. (#co-first authors)

“Structure of bile acid transporter NTCP crucial for hepatitis B virus entry”

Nature 606(7916):1021-1026 (2022)

DOI: DOI: 10.1038/s41586-022-04845-4.PubMed ID: 35580629

230) Park JH, Iwamoto M, Yun JH, Uchikubo-Kamo T, Son D, Jin Z, Yoshida H, Ohki M, Ishimoto N, Mizutani K, Oshima M, Muramatsu M, Wakita T, Shirouzu M, Liu K, Uemura T, Nomura N, Iwata S, Watashi K, Tame JRH, Nishizawa T, *Lee W and * Park SY.

“Structural insights into the HBV receptor and bile acid transporter NTCP”

Nature 606(7916):1027-1031 (2022)

DOI: DOI: 10.1038/s41586-022-04857-0.PubMed ID: 35580630

229) Maestre-Reyna M, Yang CH, Nango E, Huang WC, Ngurah Putu EPG, Wu WJ, Wang PH, Franz-Badur S, Saft M, Emmerich HJ, Wu HY, Lee CC, Huang KF, Chang YK, Liao JH, Weng JH, Gad W, Chang CW, Pang AH, Sugahara M, Owada S, Hosokawa Y, Joti Y, Yamashita A, Tanaka R, Tanaka T, Luo F, Tono K, Hsu KC, Kiontke S, Schapiro I, Spadaccini R, Royant A, Yamamoto J, Iwata S, *Essen LO, *Bessho Y, *Tsai MD.

“Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme”

Nat Chem. 14(6):677-685 (2022)

DOI: DOI: 10.1038/s41557-022-00922-3.PubMed ID: 35393554

228)Hosaka T, Nomura T, Kubo M, Nakane T, Fangjia L, Sekine SI, Ito T, Murayama K, Ihara K, Ehara H, Kashiwagi K, Katsura K, Akasaka R, Hisano T, Tanaka T, Tanaka R, Arima T, Yamashita A, Sugahara M, Naitow H, Matsuura Y, Yoshizawa S, Tono K, Owada S, Nureki O, Kimura-Someya T, Iwata S, *Nango E, *Shirouzu M.

“Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers”

Proc Natl Acad Sci U S A.119(9):e2117433119.(2022)

DOI: DOI: 10.1073/pnas.2117433119.PubMed ID: 35197289

227)Matsuoka R, *Fudim R, Jung S, Zhang C, Bazzone A, Chatzikyriakidou Y, Robinson CV, Nomura N, Iwata S, Landreh M, Orellana L, *Beckstein O. and *Drew D.

“Structure, mechanism and lipid-mediated remodeling of the mammalian Na+/H+ exchanger NHA2”

Nat Struct Mol Biol.29(2):108-120 (2022)

DOI: DOI: 10.1038/s41594-022-00738-2.PubMed ID: 35173351

226) Kishi K, Kim YS, Fukuda M, Inoue M, Kusakizako T, Wang PY, Ramakrishnan C, Byrne EFX, Thadhani E, Paggi JM, Matsui T, Yamashita K, Nagata T, Konno M, Quirin S, Lo M, Benster T, Uemura T, Liu K, Shibata M, Nomura N, Iwata S, Nureki O, Dror RO, Inoue K, *Deisseroth K and *Kato H.

“Structural basis for channel conduction in the pump-like channelrhodopsin ChRmine”

Cell 185:1-18 (2022)

DOI: DOI: 10.1016/j.cell.2022.01.007.PubMed ID: 35114111

2021

225) Pan D, Oyama R, Sato T, Nakane T, Mizunuma R, Matsuoka K, Joti Y, Tono K, Nango E, Iwata S, Nakatsu T and *Kato H.

“Crystal structure of CmABCB1 multi-drug exporter in lipidic mesophase revealed by LCP-SFX”

IUCrJ. 9(1) (2021)

DOI: DOI: 10.1107/S2052252521011611.PubMed ID: 35059217

224) *Katayama K, Suzuki K, Suno R, Kise R, Tsujimoto H, Iwata S, Inoue A, Kobayashi T & *Kandori H.

“Vibrational spectroscopy analysis of ligand efficacy in human M2 muscarinic acetylcholine receptor (M2R)”

Communications Biology.4:1321 (2021)

DOI: DOI: 10.1038/s42003-021-02836-1.PubMed ID: 34815515

223) *Murakaw T, Suzuki M, Arima T, Sugahara M, Tanaka T, Tanaka R, Iwata S, Nango E, Tono K, Hayashi H, Fukui K, Yano T, Tanizawah K and *Okajima T.

“Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase”

Acta Crystallographica Section F. 77 (2021)

DOI: DOI: 10.1107/S2053230X21008967.PubMed ID: 34605440

222) Ghilarov D, Inaba-Inoue S, Stepien P, Qu F, Michalczyk E, Pakosz Z, Nomura N, Ogasawara S, Walker GC, Rebuffat S, Iwata S, *Heddle JG and *Beis K.

“Molecular mechanism of SbmA, a promiscuous transporter exploited by antimicrobial peptides”

Science Advances 7(37): eabj5363 (2021)

DOI: DOI: 10.1126/sciadv.abj5363.PubMed ID: 34516884

221) Neville SL, Sjohamn J, Watts JA, MacDermott-Opeskin H, Fairweather SJ, Ganio K, Carey Hulyer A, McGrath AP, Hayes AJ, Malcolm TR, Davies MR, Nomura N, Iwata S, O'Mara ML, *Maher MJ and *McDevitt CA.

“The structural basis of bacterial manganese import”

Science Advance 7(32); eabg3980　(2021)

DOI: DOI: 10.1126/sciadv.abg3980.PubMed ID: 34362732

220)Cellini A, Yuan Wahlgren W, Henry L, Pandey S, Ghosh S, Castillon L, Claesson E, Takala H, Kubel J, Nimmrich A, Kuznetsova V, Nango E, Iwata S, Owada S, Stojkovi? EA, Schmidt M, Ihalainen JA and Westenhoff S.

“The three-dimensional structure of Drosophila melanogaster (6-4) photolyase at room temperature”

Acta Crystallogr D Struct Biol. 77(Pt 8):1001-1009 (2021)

DOI: DOI: 10.1107/S2059798321005830.PubMed ID: 34342273

219) Butryn A, Simon PS, Aller P, Hinchliffe P, Massad RN, Leen G, Tooke CL, Bogacz I, Kim IS, Bhowmick A, Brewster AS, Devenish NE, Brem J, Kamps JJAG, Lang PA, Rabe P, Axford D, Beale JH, Davy B, Ebrahim A, Orlans J, Storm SLS, Zhou T, Owada S, Tanaka R, Tono K, Evans G, Owen RL, Houle FA, Sauter NK, Schofield CJ, Spencer J, Yachandra VK, Yano J, Kern JF and Orville AM.

“An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography”

Nat Commun.12(1):4461 (2021)

DOI: DOI: 10.1038/s41467-021-24757-7.PubMed ID: 34294694

218) Okamoto H.H, Miyauchi H, Inoue A, Raimondi F, Tsujimoto H, Kusakizako T, Shihoya W, Yamashita K, Suno R, Nomura N, Kobayashi T, Iwata S, Nishizawa T and Nureki O.

“Cryo-EM structure of the human MT1-Gi signaling complex”

Nat Struct Mol Biol.28: 694-701 (2021)

DOI: DOI: 10.1038/s41594-021-00634-1.PubMed ID: 34354246

217) Maeda S, Shiimura Y, Asada H, Hirata K, Luo F, Nango E, Tanaka N, Toyomoto M, Inoue A, Aoki J, *Iwata S and *Hagiwara M.

“Endogenous agonist-bound S1PR3 structure reveals determinants of G protein-subtype bias”

Science Adv. 7: eabf5325 (2021)

DOI: DOI: 10.1126/sciadv.abf5325.PubMed ID: 34108205

216) Jin F, Sun M, Fujii T, Yamada Y, Wang J, Maturana AD, Wada M, Su S, Ma J, Takeda H, Kusakizako T, Tomita A, Nakada-Nakura Y, Liu K, Uemura T, Nomura Y, Nomura N, Ito K, Nureki O, Namba K, Iwata S, Yu Y and Hattori M.

“The structure of MgtE in the absence of magnesium provides new insights into channel gating”

PLoS Biol. 19(4):e3001231 (2021)

DOI: DOI: 10.1371/journal.pbio.3001231.PubMed ID: 33905418

215) Li H, Nakajima Y, Nomura T, Sugahara M, Yonekura S, Chan SK, Nakane T, Yamane T, Umena Y, Suzuki M, Masuda T, Motomura T, Naitow H, Matsuura Y, Kimura T, Tono K, Owada S, Joti Y, Tanaka R, Nango E, Akita F, Kubo M, Iwata S, Shen JR and Suga M.

“Capturing structural changes of the S 1 to S 2 transition of photosystem II using time-resolved serial femtosecond crystallography”

IUCrJ. 8(Pt 3):431-443. (2021)

DOI: DOI: 10.1107/S2052252521002177.PubMed ID: 33953929

214) Oda K, Nomura T, Nakane T, Yamashita K, Inoue K, Ito S, Vierock J, Hirata K, Maturana AD, Katayama K, Ikuta T, Ishigami I, Izume T, Umeda R, Eguma R, Oishi S, Kasuya G, Kato T, Kusakizako T, Shihoya W, Shimada H, Takatsuji T, Takemoto M, Taniguchi R, Tomita A, Nakamura R, Fukuda M, Miyauchi H, Lee Y, Nango E, Tanaka R, Tanaka T, Sugahara M, Kimura T, Shimamura T, Fujiwara T, Yamanaka Y, Owada S, Joti Y, Tono K, Ishitani R, Hayashi S, Kandori H, Hegemann P, Iwata S, Kubo M, Nishizawa T and Nureki O.

“Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin”

eLife. 10:e62389.(2021)

DOI: DOI: 10.7554/eLife.62389.PubMed ID: 33752801

213) Carrillo M, Pandey S, Sanchez J, Noda M, Poudyal I, Aldama L, Malla TN, Claesson E, Wahlgren WY, Feliz D, Šrajer V, Maj M, Castillon L, Iwata S, Nango E, Tanaka R, Tanaka T, Fangjia L, Tono K, Owada S, Westenhoff S, Stojković EA and Schmidt M.

“High-resolution crystal structures of transient intermediates in the phytochrome photocycle”

Structure. March 22, 2021 ［Online ahead of print.］

DOI: DOI: 10.1016/j.str.2021.03.004.PubMed ID: 33756101

212) Toyomoto M, Inoue A, Iida K, Denawa M, Kii I, Ngako Kadji FM, Kishi T, Im D, Shimamura T, Onogi H, Yoshida S, Iwata S, Aoki J, Hosoya T and Hagiwara M.

“S1PR3-G 12-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation”

Cell Chem Biol. 2021 Feb 8.［Online ahead of print.］

DOI: DOI: 10.1016/j.chembiol.2021.01.004.PubMed ID: 33561428

2020

211) Im D, Inoue A, Fujiwara T, Nakane T, Yamanaka Y, Uemura T, Mori C, Shiimura Y, Kimura KT, Asada H, Nomura N, Tanaka T, Yamashita A, Nango E, Tono K, Kadji FMN, Aoki J, Iwata S and Shimamura T.

“Structure of the dopamine D2 receptor in complex with the antipsychotic drug spiperone”

Nat Commun.11(1):6442 (2020)

DOI: DOI: 10.1038/s41467-020-20221-0.PubMed ID: 33353947

210) Nojima S, Fujita Y, Kimura KT, Nomura N, Suno R, Morimoto K, Yamamoto M, Noda T, Iwata S, Shigematsu H and Kobayashi T.

“Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein”

Structure.29(3):252-260 (2020)

DOI: DOI: 10.1016/j.str.2020.11.007.PubMed ID: 33264604

209) Deshpande C.N., Azucenas C. R., Qiao B, Nomura N, Xin V, Font J, Iwata S, Ganz T, Nemeth E, Mackenzie B. and Jormakka M.

“Isolation and thermal stabilization of mouse ferroportin”

FEBS Open Bio.11(1):26-34 (2020)

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208) Ihara K, Hato M, Nakane T, Yamashita K, Kimura-Someya T, Hosaka T, Ishizuka-Katsura Y, Tanaka R, Tanaka T, Sugahara M, Hirata K, Yamamoto M, Nureki O, Tono K, Nango E, Iwata S and Shirouzu M.

“Isoprenoid-chained lipid EROCOC17+4: a new matrix for membrane protein crystallization and a crystal delivery medium in serial femtosecond crystallography”

Sci Rep. 10(1):19305 (2020)

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207)Matoba K, Kotani T, Tsutsumi A, Tsuji T, Mori T, Noshiro D, Sugita Y, Nomura N, Iwata S, Ohsumi Y, Fujimoto T, Nakatogawa H, Kikkawa M and Noda NN.

“Atg9 is a lipid scramblase that mediates autophagosomal membrane expansion”

Nat Struct Mol Biol.27(12): 1185-1193 (2020)

DOI: DOI: 10.1038/s41594-020-00518-w.PubMed ID: 33106658

206) Shiimura Y, Horita S, Hamamoto A, Asada H, Hirata K, Tanaka M, Mori K, Uemura T, Kobayashi T, Iwata S and Kojima M.

“Structure of an antagonist-bound ghrelin receptor reveals possible ghrelin recognition mode”

Nat Commun.11(1):4160 (2020)

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205)Miyagi H, Asada H, Suzuki M, Takahashi Y, Yasunaga M, Suno C, Iwata S, Saito JI.

“The discovery of a new antibody for BRIL-fused GPCR structure determination”

Sci Rep. 10(1):11669. (2020)

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204) C. Wickstrand , G. Katona , T.i Nakane, P. Nogly , J. Standfuss , E. Nango and R. Neutze.

“A tool for visualizing protein motions in time-resolved crystallography”

Struct Dyn. 7, 024701 (2020)

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203) Umeda R, Satouh Y, Takemoto M, Nakada-Nakura Y, Liu K, Yokoyama T, Shirouzu M, Iwata S, Nomura N, Sato K, Ikawa M, Nishizawa T, Nureki O.

“Structural insights into tetraspanin CD9 function.”

Nat Commun. 11(1):1606 (2020)

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202) Claesson E, Wahlgren WY, Takala H, Pandey S, Castillon L, Kuznetsova V, Henry L, Panman M, Carrillo M, Kubel J, Nanekar R, Isaksson L, Nimmrich A, Cellini A, Morozov D, Maj M, Kurttila M, Bosman R, Nango E, Tanaka R, Tanaka T, Fangjia L, Iwata S, Owada S, Moffat K, Groenhof G, A Stojkovi? E, A Ihalainen J, Schmidt M, Westenhoff S.

“The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser.”

eLife 2020;9:e53514 (2020)

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201) Wolff AM, Young ID, Sierra RG, Brewster AS, Martynowycz MW, Nango E, Sugahara M, Nakane T, Ito K, Aquila A, Bhowmick A, Biel JT, Carbajo S, Cohen AE, Cortez S, Gonzalez A, Hino T, Im D, Koralek JD, Kubo M, Lazarou TS, Nomura T, Owada S, Samelson AJ, Tanaka T, Tanaka R, Thompson EM, van den Bedem H, Woldeyes RA, Yumoto F, Zhao W, Tono K, Boutet S, Iwata S, Gonen T, Sauter NK, Fraser JS and Thompson MC.

“Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals.”

IUCrJ. 7(Pt 2):306-323. (2020)

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2019

200) Asada H, Inoue A, Ngako Kadji FM, Hirata K, Shiimura Y, Im D, Shimamura T, Nomura N, Iwanari H, Hamakubo T, Kusano-Arai O, Hisano H, Uemura T, Suno C, Aoki J, Iwata S.

“The Crystal Structure of Angiotensin II Type 2 Receptor with Endogenous Peptide Hormone.”

Structure.28: 1-8 (2019)

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199) Ishchenko A, Stauch B, Han GW, Batyuk A, Shiriaeva A, Li C, Zatsepin N, Weierstall U, Liu W, Nango E, Nakane T, Tanaka R, Tono K, Joti Y, Iwata S, Moraes I, Gati C and Cherezov V.

“Toward G protein-coupled receptor structure-based drug design using X-ray lasers.”

IUCrJ.6(Pt 6):1106-1119. (2019)

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198) Katayama K, Suzuki K, Suno R, Tsujimoto H, Iwata S, Kobayashi T, Kandori H.

“Ligand Binding-Induced Structural Changes in the M2 Muscarinic Acetylcholine Receptor Revealed by Vibrational Spectroscopy.”

J Phys Chem Lett.10(22)：7270-7276 (2019)

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197) Shimazu Y, Tono K, Tanaka T, Yamanaka Y, Nakane T, Mori C, Terakado Kimura K, Fujiwara T, Sugahara M, Tanaka R, Doak RB, Shimamura T, Iwata S, Nango E and Yabashi M.

“High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure”

J. Appl. Cryst. 52：1280-1288 (2019)

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“Improvement of Production and Isolation of Human Neuraminidase-1 in Cellulo Crystals”

ACS Appl. Bio Mater.2(11): 4941-4952 (2019)

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"Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers."

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"High-resolution crystal structures of a myxobacterial phytochrome at cryo and room temperatures."

Struct Dyn.6(5):054701 (2019)

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193) Kinjo T, Terai K, Horita S, Nomura N, Sumiyama K, Togashi K, Iwata S, Matsuda M.

"FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics."

Nat Methods.16: 1029-1036 (2019)

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192)Wickstrand C, Nogly P, Nango E, Iwata S, Standfuss J and Neutze R.

“Bacteriorhodopsin: Structural Insights Revealed Using X-Ray Lasers and Synchrotron Radiation. ”

Annu Rev Biochem.88.14.1-14.25 (2019)

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191)Terakado-Kimura K, Asada H, Inoue A, Kadji FMN, Im D, Mori C, Arakawa T, Hirata K, Nomura Y, Nomura N, Aoki J, Iwata S and Shimamura T.

“Structures of the 5-HT2A receptor in complex with the antipsychotics risperidone and zotepine”

Nat.Struct.Mol.Biol. 26: 121-128 (2019)

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Crystal structure of the endogenous agonist-bound prostanoid receptor EP3

Nat Chem Biol.15: 8-10 (2019)

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Ligand binding to human prostaglandin E receptor EP4 at the lipid-bilayer interface

Nat Chem Biol.15: 18-26 (2019)

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2018

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Structural insights into the subtype-selective antagonist binding to the M2 muscarinic receptor

Nat Chem Biol.14: 1150-1158 (2018)

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187) Nagarathinam K, Nakada-Nakura Y, Parthier C, Terada T, Juge N, Jaenecke F, Liu K, Hotta Y, Miyaji T, Omote H, Iwata S, Nomura N, Stubbs MT, Tanabe M.

Outward open conformation of a Major Facilitator Superfamily multidrug/H+antiporter provides insights into switching mechanism.

Nat Commun. 9(1):4005. (2018)

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Positive/Negative Allosteric Modulation Switching in an Umami Taste Receptor (T1R1/T1R3) by a Natural Flavor Compound, Methional.

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“Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station "KIBO″.”

Anal Biochem.559: 91-93 (2018)

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184) Asada H, Horita S, Hirata K, Shiroishi M, Shiimura Y, Iwanari H, Hamakubo T, Shimamura T, Nomura N, Kusano-Arai O, Uemura T, Suno C, Kobayashi T and Iwata S.

“Crystal structure of the human angiotensin II type 2 receptor bound to an angiotensin II analog”

Nat.Struct.Mol.Biol.25, 570-576 (2018)

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Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser

Science 361(6398): eaat0094 (2018)

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Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy.

Biochem Biophys Rep. 14: 58-63 (2018)

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181) Ono M, Horita S, Sato Y, Nomura Y, Iwata S, Nomura N.

Structural basis for tumor necrosis factor blockade with the therapeutic antibody golimumab.

Protein Sci. 27(6): 1038-1046 (2018)

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180) Suzuki N, Takamuku Y, Asakawa T, Inai M, Hino T, Iwata S, Kan T. and Murata T.

An efficient screening method for purifying and crystallizing membrane proteins using modified clear-native PAGE.

Anal Biochem.548: 7-14 (2018)

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179) Yamashita T, Inaoka DK, Shiba T, Oohashi T, Iwata S, Yagi T, Kosaka H, Miyoshi H, Harada S, Kita K. and Hirano K.

Ubiquinone binding site of yeast NADH dehydrogenase revealed by structures binding novel competitive- and mixed-type inhibitors.

Sci Rep.8(1):2427 (2018)

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178) Suno R, Terakado-Kimura K, Nakane T, Yamashita K, Wang J, Fujiwara T, Yamanaka Y, Im D, Horita S, Tsujimoto H, Tawaramoto-Sasanuma M, Hirokawa T, Nango E, Tono K, Kameshima T, Hatsui T, Joti Y, Yabashi M, Shimamoto K, Yamamoto M, Rosenbaum DM, Iwata S, Shimamura T, and Kobayashi T.

Crystal Structures of Human Orexin 2 Receptor Bound to the Subtype-Selective Antagonist EMPA

Structure 26, 1-13(2018)

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2017

177) Gorel A, Motomura K, Fukuzawa H, Doak RB, Grunbein ML, Hilpert M, Inoue I, Kloos M, Nass Kovacs G, Nango E, Nass K, Roome CM, Shoeman RL, Tanaka R, Tono K, Foucar L, Joti Y, Yabashi M, Iwata S, Ueda K, Barends TRM, Schlichting I.

Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing.

Sci Data. 4:170188. (2017)

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Serial femtosecond crystallography at the SACLA: breakthrough to dynamic structural biology.

Biophys Rev.8(1):1585 (2017)

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175) Sanchez-Gonzalez A, Johnson AS, Fitzpatrick A, Hutchison CDM, Fare C, Cordon-Preciado V, Dorlhiac G, Ferreira J, Morgan RML, Marangos JP, Owada S, Nakane T, Tanaka R, Tono K, Iwata S, and van Thor JJ.

Coincidence timing of femtosecond optical pulses in an X-ray free electron laser

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Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate.

Nat Commun. 8(1):1585 (2017)

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173) Gorel A, Motomura K, Fukuzawa H, Doak RB, Grunbein ML, Hilpert M, Inoue I, Kloos M, Kovacsova G, Nango E, Nass K, Roome CM, Shoeman RL, Tanaka R, Tono K, Joti Y, Yabashi M, Iwata S, Foucar L, Ueda K, Barends TRM, Schlichting I.

Multi-wavelength anomalous diffraction de novo phasing using a two-colour X-ray free-electron laser with wide tunability.

Nat Commun. 8(1):1170.(2017)

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X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature.

Int J Mol Sci.18(9): 1918 (2017)

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Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA

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Experimental phase determination with seleno- methionine or mercury-derivatization in serial femtosecond crystallography

IUCrJ. 4(5); 639-647 (2017)

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XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction.

PNAS.114(51):13357-13362 (2017)

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Structural insights into ligand recognition by the lysophosphatidic acid receptor LPA6

Nature 548; 356-360 (2017)

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Protein-ligand complex structureand comparison with structures from synchrotron radiation.

Acta Cryst. D 73: 702-709 (2017)

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Serial femtosecond crystallography structure of cytochrome c oxidase at room temperature.

Sci Rep.7(1):4518 (2017)

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165) Nagarathinam K, Jaenecke F, Nakada-Nakura Y, Hotta Y, Liu K, Iwata S, Stubbs MT, Nomura N and Tanabe M.

The multidrug-resistance transporter MdfA from Escherichia coli:crystallization and X-ray diffraction analysis

Acta Cryst. F 73: 423-430 (2017)

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Hot-Spot Residues to be Mutated Common in G Protein-Coupled Receptors of Class A: Identification of Thermostabilizing Mutations Followed by Determination of Three-Dimensional Structures for Two Example Receptors.

J Phys Chem B. 121(26); 6341- 6350 (2017)

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Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL.

Nature 543;131-135 (2017)

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2016

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A three-dimensional movie of structural changes in bacteriorhodopsin.

Science. 354(6319);1552-1557(2016)

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Cell Res. 26(12); 1288-1301(2016)

DOI:10.1038/cr.2016.140. PubMed ID: 27909292

156) Nakane T, Hanashima S, Suzuki M, Saiki H, Hayashi T, Kakinouchi K, Sugiyama S, Kawatake S, Matsuoka S, Matsumori N, Nango E, Kobayashi J, Shimamura T, Kimura K, Mori C, Kunishima N, Sugahara M, Takakyu Y, Inoue S, Masuda T, Hosaka T, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Inoue T, Nureki O , Iwata S, Murata M, and Mizohata E.

Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent

PNAS 113(46); 13039-13044 (2016)

DOI:10.1073/pnas.1602531113 PubMed ID: 27799539

155) Suno, R., Asada, H. and Kobayashi,T.

Towards the Crystal Structure Determination of Muscarinic Acetylcholine Receptors.

Springer 107: 1-13 (2016)

DOI:10.1007/978-1-4939-2858-3_1

154) Horita, S., Nomura, Y., Sato, Y., Shimamura, T., Iwata, S. and Nomura, N.

High-resolution crystal structure of the therapeutic antibody pembrolizumab bound to the human PD-1

Scientific Reports(13) 6: 35297 (2016)

DOI:10.1038/srep35297 PubMed ID: 27734966

153) Kabe Y, Yamamoto T, Kajimura M, Sugiura Y, Koike I, Ohmura M, Nakamura T, Tokumoto Y, Tsugawa H, Handa H, Kobayashi T and Suematsu M.

Cystathionine β-synthase and PGRMC1 as CO sensors.

Free Radic Biol Med. 99; 333-344 (2016)

DOI:10.1016/j.freeradbiomed.2016.08.025 PubMed ID: 27565814

152) Nomura Y, Sato Y, Suno R, Horita S, Iwata S, Nomura N.

The intervening removable affinity tag (iRAT) production system facilitates Fv antibody fragment-mediated crystallography.

Protein Science 25 (12); 2268-2276 (2016)

DOI:10.1002/pro.3035 PubMed ID: 27595817

151) Nogly P, Panneels V, Nelson G, Gati C, Kimura T, Milne C, Milathianaki D, Kubo M, Wu W, Conrad C, Coe J, Bean R, Zhao Y, Bath P, Dods R, Harimoorthy R, Beyerlein KR, Rheinberger J, James D, DePonte D, Li C, Sala L, Williams GJ, Hunter MS, Koglin JE, Berntsen P,Nango E, Iwata S, Chapman HN, Fromme P, Frank M, Abela R, Boutet S, Barty A, White TA, Weierstall U, Spence J, Neutze R,Schertler G,and Standfuss J.

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Nat. Commun.7, 12314 (2016)

DOI:10.1038/ncomms12314 PubMed ID: 27545823

150) Nakane T, Joti Y, Tono K, Yabashi M, Nango E, Iwata S, Ishitania R. and Nureki O.

Data processing pipeline for serial femtosecond crystallography at SACLA

J. Appl. Cryst. 49(3); 1035-1041 (2016)

DOI:10.1107/S1600576716005720 PubMed ID: 27275146

149) Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD and Byrne B.

Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.

Nat Commun. 7; 11336 (2016)

DOI:10.1038/ncomms11336. PubMed ID: 27088252

148) Sugahara M, Song C, Suzuki M, Masuda T, Inoue S, Nakane T, Yumoto F, Nango E, Tanaka R, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Nureki O, Numata K and Iwata S.

Oil-free hyaluronic acid matrix for serial femtosecond crystallography

Scientific Reports 6; 24484 (2016)

DOI:10.1038/srep24484 PubMed ID: 27087008

147) Reithmeier RA, Casey JR, Kalli A, Sansom MS, Alguel Y and Iwata S.

Band 3, the Human Red Cell Chloride/Bicarbonate Anion Exchanger (AE1, SLC4A1), in a Structural Context

Biochim Biophys Acta.1858(7); 1507-1532 (2016)

DOI:10.1016/j.bbamem.2016.03.030. PubMed ID: 27058983

146) Mafune F, Miyajima K, Tono K, Takeda Y, Kohno J, Miyauchi N, Kobayashi J, Joti Y, Nango E, Iwata S and Yabashi Y.

Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography

Acta Cryst. D72; 520-523 (2016)

DOI:10.1107/S2059798316001480 PubMed ID: 27050131

145) Kabe Y, Nakane T, Koike I, Yamamoto T, Sugiura Y, Harada E, Sugase K, Shimamura T, Ohmura M, Muraoka K, Yamamoto A, Uchida T, Iwata S, Yamaguchi Y, Krayukhina E, Noda M, Handa H, Ishimori K, Uchiyama S, Kobayashi T and Suematsu M.

Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance

Nat Commun. 7(11030)

DOI:10.1038/ncomms11030 PubMed ID: 26988023

144) Fukuda Y, Tse KM, Nakane T, Nakatsu T, Suzuki M, Sugahara M,Inoue S, Masuda T, Yumoto F, Matsugaki N, Nango E, Tono K, Joti Y, Kameshima T, Song C, Hatsui T, Yabashi M, Nureki O, Murphy ME, Inoue T, Iwata S, and Mizohata E.

Redox-coupled proton transfer mechanism in nitrite reductase revealed by femtosecond crystallography

PNAS 113(11); 2928-2933 (2016)

DOI:10.1073/pnas.1517770113 PubMed ID: 26929369

143) Fukuda, Y., Tse, KM., Suzuki, M., Diederichs, K., Hirata, T., Nakane, T., Sugahara, M., Nango, E., Tono, K., Joti, Y., Kameshima, T., Song, C., Hatsui, T., Yabashi, M., Nureki, O., Matsumura, H., Inoue, T., Iwata, S. and Mizohata, E.

Redox-coupled structural changes in nitrite reductase revealed by serial femtosecond and microfocus crystallography

J. Biochem. 159(5); 527-538 (2016)

DOI:10.1093/jb/mvv133 PubMed ID: 26769972

2015

142) Nakane,T., Song, C., Suzuki, M., Nango, E., Kobayashi, J., Masuda, T., Inoue, S., Mizohata, E., Nakatsu, T., Tanaka, T., Tanaka, R., Shimamura, T., Tono, K., Joti, Y., Kameshima, T., Hatsui, T., Yabashi, M., Nureki, O., Iwata, S. and Sugahara, M.

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography

Acta Cryst. D71(Pt12); 2519-2525 (2015)

DOI:10.1107/S139900471501857X PubMed ID: 26627659

141) Arakawa T, Kobayashi-Yurugi T, Alguel Y, Iwanari H, Hatae H, Iwata M, Abe Y, Hino T, Ikeda-Suno C, Kuma H, Kang D, Murata T, Hamakubo T, Cameron AD, Kobayashi T, Hamasaki N, Iwata S.

Crystal structure of the anion exchanger domain of human erythrocyte band 3.

Science 350(6261): 680-684 (2015)

DOI:10.1126/science.aaa4335. PubMed ID: 26542571

140) Nomura, N., Verdon, G,. Kang, HJ., Shimamura, T., Nomura, Y., Sonoda, Y., Hussien, SA., Qureshi, AA., Coincon, M., Sato, Y., Abe, H., Nakada-Nakura, Y., Hino, T., Arakawa, T., Kusano-Arai, O., Iwanari, H., Murata, T., Kobayashi, T., Hamakubo, T., Kasahara, M., Iwata, S. and Drew, D.

Structure and mechanism of the mammalian fructose transporter GLUT5.

Nature 526; 397-401（2015)

DOI:10.1038/nature14909. PubMed ID: 26416735

139) Yamashita, K., Pan, D., Okuda, T., Sugahara, M., Kodan, A., Yamaguchi, T., Murai, T., Gomi, K., Kajiyama, N., Mizohata, E., Suzuki, M., Nango, E., Tono, K., Joti, Y., Kameshima, T., Park, J., Song, C., Hatsui, T., Yabashi, M., Iwata, S., Kato, H., Ago, H., Yamamoto, M. and Nakatsu, T.

An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography

Sci Rep.5 :14017 (2015)

DOI:10.1038/srep14017 PubMed ID: 26360462

138) Axford, D., Foadi, J., Hu, NJ., Choudhury, HG., Iwata, S., Beis, K., Evans, G. and Alguel, Y.

Structure determination of an integral membrane protein at room temperature from crystals in situ.

Acta Crystallogr D Biol Crystallogr. 71(Pt 6); 1228-1237 (2015)

DOI:10.1107/S139900471500423X. PubMed ID: 26057664

137) Tono K, Nango E, Sugahara M, Song C, Park J, Tanaka T, Tanaka R, Joti Y, Kameshima T, Ono S, Hatsui T, Mizohata E, Suzuki M, Shimamura T, Tanaka Y, Iwata S, Yabashi M.

Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser.

J Synchrotron Radiat. 1; 22(Pt 3); 532-537 (2015)

DOI:10.1107/S1600577515004464 PubMed ID: 25931065

136) Tanabe, H., Fujii, Y., Okada-Iwabu, M., Iwabu, M., Nakamura, Y., Hosaka, T., Motoyama, K., Ikeda, M., Wakiyama, M., Terada, T., Ohsawa, N., Hato, M., Ogasawara, S., Hino, T., Murata, T., Iwata, S., Hirata, K., Kawano, Y., Yamamoto, M., Kimura-Someya, T., Shirouzu, M., Yamauchi, T., Kadowaki, T. and Yokoyama, S.

Crystal structures of the human adiponectin receptors

Nature. 520; 312-316 (2015)

DOI:10.1038/nature14301 PubMed ID: 25855295

135) Shiroishi, M., Kobayashi,T.

Screening of Stable G-Protein-Coupled Receptor Variants in Saccharomyces cerevisiae.

Methods Mol Biol. 1261;159-170 (2015)

DOI:10.1007/978-1-4939-2230-7_9 PubMed ID: 25502199

134) Tanabe, H., Motoyama, K., Ikeda, M., Wakiyama, M., Terada, T., Ohsawa, N., Hosaka, T., Hato, M., Fujii, Y., Nakamura, Y., Ogasawara, S., Hino, T., Murata, T., Iwata, S., Okada-Iwabu, M., Iwabu, M., Hirata, K., Kawano, Y., Yamamoto, M., Kimura-Someya, T., Shirouzu, M., Yamauchi, T., Kadowaki, T. and Yokoyama, S.

Expression, purification, crystallization, and preliminary X-ray crystallographic studies of the human adiponectin receptors, AdipoR1 and AdipoR2.

J Struct Funct Genomics. 16(1); 11-23 (2015)

DOI:10.1007/s10969-014-9192-z PubMed ID: 25575462

133) Hu, N-Jen., Rada, H., Rahman, N., Nettleship, J.E., Bird, L., Iwata, S., Drew, D., Cameron, A.D., and Owens, R.J.

GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System.

Springer. 1261 (3) 197-209 (2015)

DOI:10.1007/978-1-4939-2230-7_11 PubMed ID: 25502201

132) Sugahara,M., Mizohata,E., Nango,E., Suzuki,M., Tanaka,T., Masuda,T., Tanaka,T., Shimamura,T., Tanaka,Y., Suno,C., Ihara,K., Pan,D., Kakinouchi,K., Sugiyama,S., Murata,M., Inoue,T., Tono,K., Song,C., Park,J., Kameshima,T., Hatsui,T., Joti,Y., Yabashi,M., Iwata,S.

Grease matrix as a versatile carrier of proteins for serial crystallography

Nature Methods 12, 61-63 (2015)

DOI:10.1038/nmeth.3172 PubMed ID: 25384243

2014

131)  Suharni, Nomura, Y., Arakawa, T., Hino, T., Abe, H., Nakada-Nakura, Y., Sato, Y., Iwanari, H., Shiroishi, M., Asada, H., Shimamura, T., Murata, T., Kobayashi, T., Hamakubo, T., Iwata, S. and Nomura, N.

Proteoliposome-based selection of a recombinant antibody fragment against the human M2 muscarinic acetylcholine receptor.

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33(6); 378-385(2014)

DOI:10.1089/mab.2014.0041 PubMed ID: 25545206

130) Lee, C., Yashiro, S., Dotson, DL., Uzdavinys, P., Iwata, S., Sansom, MS., von Ballmoos, C., Beckstein, O., Drew, D. and Cameron, A.D.

Crystal structure of the sodium-proton antiporter NhaA dimer and new mechanistic insights.

J.Gen.Physiol. 144 (6); 529-544 (2014)

DOI:10.1085/jgp.201411219 PubMed ID: 25422503

129) Adachi, M., Kawasaki, A., Nojima, H., Nishida, E., Tsukita, S.

Involvement of IQGAP family proteins in the regulation of mammalian cell cytokinesis

Genes to Cells 19(11); 803-820(2014)

DOI:10.1111/gtc.12179 PubMed ID: 25229330

128) Koike-Takeshita, A., Arakawa, T., Taguchi, H., Shimamura,T.

Crystal structure of a symmetric football-shaped GroEL:GroES2complex determined at 3.8Å reveals rearrangement between two GroEL rings

J Mol Biol.426(21); 3634-3641(2014)

DOI:10.1016/j.jmb.2014.08.017. PubMed ID: 25174333

127)  Simmons, KJ., Jackson, SM., Brueckner, F., Patching, SG., Beckstein, O., Ivanova, E., Geng, T., Weyand, S., Drew, D., Lanigan, J., Sharples, DJ., Sansom, MS., Iwata, S., Fishwick, CW., Johnson, AP., Cameron, AD., Henderson, PJ.

Molecular mechanism of ligand recognition by membrane transport protein, Mhp1

EMBO. 33(12); 1285-1415 (2014)

DOI:10.15252/embj.201387557. PubMed ID: 24952894

126)  Choudhury, HG., Tong, Z., Mathavan, I., Li, Y., Iwata, S., Zirah, S., Rebuffat, S., van Veen, HW., Beis, K.

Structure of an antibacterial peptide ATP-binding cassette transporter in a novel outward occluded state

PNAS. 111(25); 9145-9150 (2014)

DOI:10.1073/pnas.1320506111. PubMed ID: 24920594

125)  Gallat, FX., Matsugaki, N., Coussens, NP., Yagi, KJ., Boudes, M., Higashi, T., Tsuji, D., Tatano, Y., Suzuki, M., Mizohata, E., Tono, K., Joti, Y., Kameshima, T., Park, J., Song, C., Hatsui, T., Yabashi, M., Nango, E., Itoh, K., Coulibaly, F., Tobe, S., Ramaswamy, S., Stay, B., Iwata, S., Chavas, LM.

In vivo crystallography at X-ray free-electron lasers: the next generation of structural biology?

Phil. Trans. R. Soc. B. 369(1647); 20130497 (2014)

DOI:10.1098/rstb.2013.0497. PubMed ID: 24914164

2013

124) Alam, J., Yamato, I., Arai, S., Saijo, S., Mizutani, K., Ishizuka-Katsura, Y., Ohsawa, N., Terada, T., Shirouzu, M., Yokoyama, S., Iwata, S., Kakinuma, Y., Murata, T.

Mutant LV(476-7)AA of A-subunit of Enterococcus hirae V1-ATPase: High affinity of A3B3 complex to DF axis and low ATPase activity.

Springerplus. 2; 689 (2013)

DOI:10.1186/2193-1801-2-689 PubMed ID: 24404436

123) Manolaridis, I., Kulkarni, K., Dodd, RD., Ogasawara, S., Zhang, Z., Bineva, G., O’Reilly, N., Hanrahan, SJ., Thompson, AJ., Cronin, N., S, Iwata., Barford, D.

Mechanism of farnesylated CAAX protein processing by the intramembrane protease Rce1.

Nature. 504(7479):301-305(2013)

DOI:10.1038/nature12754 PubMed ID: 24291792

Press Release:がんを引き起こす膜たんぱく質の立体構造と働きを解明 ～がんを抑制する薬剤の設計へ～

122) Lee C, Kang HJ, von Ballmoos C, Newstead S, Uzdavinys P, Dotson DL, Iwata S, Beckstein O, Cameron AD, Drew D.

A two-domain elevator mechanism for sodium/proton antiport.

Nature. 501(7468):573-7.(2013)

DOI:10.1038/nature12484 PubMed ID:23995679

121) Alam MJ, Arai S, Saijo S, Suzuki K, Mizutani K, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Kakinuma Y, Yamato I, Murata T.

Loose Binding of the DF Axis with the A3B3 Complex Stimulates the Initial Activity of Enterococcus hirae V1-ATPase.

PLoS One. 8(9):e74291.(2013)

DOI:10.1371/journal.pone.0074291 PubMed ID:24058539

120) Foadi J, Aller P, Alguel Y, Cameron A, Axford D, Owen RL, Armour W, Waterman DG, Iwata S, Evans G.

Clustering procedures for the optimal selection of data sets from multiple crystals in macromolecular crystallography.

Acta Crystallogr D Biol Crystallogr. 69(8); 1617-1632 (2013)

DOI:10.1107/S0907444913012274 PubMed ID:23897484

119) Murai Y, Masuda K, Ogasawara Y, Wang L, Hashidoko Y, Hatanaka Y, Iwata S, Kobayashi T, Hashimoto M.

Synthesis of Photoreactive 2-Phenethylamine Derivatives - Synthesis of Adenosine Derivatives Enabling Functional Analysis of Adenosine Receptors by Photoaffinity Labeling.

European J Org Chem. 2013(12); 2428-2433 (2013)

DOI:10.1002/ejoc.201201657

118) Rahman S, Yamato I, Saijo S, Mizutani K, Katsura-Ishizuka Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Murata T.

Biochemical and biophysical properties of interactions between subunits of the peripheral stalk region of human V-ATPase.

PloS One 8(2); 1-13 (2013)

DOI:10.1371/journal.pone.0055704 PubMed ID:23409023

117) Hino T, Iwata S, Murata T.

Generation of functional antibodies for mammalian membrane protein crystallography.

Curr Opin Struct Biol. 2013 May 8. pii: S0959-440X(13)00074-2.

DOI:10.1016/j.sbi.2013.04.007. PubMed ID:23664057

116) Arai S, Saijo S, Suzuki K, Mizutani K, Kakinuma Y, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Yamato I, Murata T.

Rotation mechanism of Enterococcus hirae V1-ATPase based on asymmetric crystal structures.

Nature. 2013 Jan 31;493(7434):703-7.

DOI:10.1038/nature11778 PubMed ID:23334411

Related PDB Entries:3VR6, 3VR5, 3VR4, 3VR3, 3VR2, 3VR1

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2012

115) Iwata M, Lee Y, Yamashita T, Yagi T, Iwata S, Cameron AD, Maher MJ.

The structure of the yeast NADH dehydrogenase (Ndi1) reveals overlapping binding sites for water- and lipid-soluble substrates.

Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15247-52.

DOI:10.1073/pnas.1210059109 PubMed ID:22949654

Related PDB Entries:4GAV, 4GAP, 4G9K

114) Suno R, Shimoyama M, Abe A, Shimamura T, Shimodate N, Watanabe YH, Akiyama Y, Yoshida M.

Conformational transition of the lid helix covering the protease active site is essential for the ATP-dependent protease activity of FtsH.

FEBS Lett. 2012 Sep 21;586(19):3117-21.

DOI:10.1016/j.febslet.2012.07.069 PubMed ID:23166924

113) Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S.

Alternating access mechanism in the POT family of oligopeptide transporters.

EMBO J. 2012 Aug 15;31(16):3411-21.

DOI:10.1038/emboj.2012.157 PubMed ID:22659829

Related PDB Entries:4APS

112) Sasaki H, Kubota K, Lee WC, Ohtsuka J, Kojima M, Iwata S, Nakagawa A, Takahashi K, Tanokura M.

The crystal structure of an intermediate dimer of aspergilloglutamic peptidase that mimics the enzyme-activation product complex produced upon autoproteolysis.

J Biochem. 2012 Jul;152(1):45-52.

DOI:10.1093/jb/mvs050 PubMed ID:22569035

Related PDB Entries:3TRS

111) Shiroishi M, Tsujimoto H, Makyio H, Asada H, Yurugi-Kobayashi T, Shimamura T, Murata T, Nomura N, Haga T, Iwata S, Kobayashi T.

Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae.

Microb Cell Fact. 2012 Jun 13;11:78.

DOI:10.1186/1475-2859-11-78 PubMed ID:22694812

110) Hino T, Arakawa T, Iwanari H, Yurugi-Kobayashi T, Ikeda-Suno C, Nakada-Nakura Y, Kusano-Arai O, Weyand S, Shimamura T, Nomura N, Cameron AD, Kobayashi T, Hamakubo T, Iwata S, Murata T.

G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody.

Nature. 2012 Jan 29;482(7384):237-40.

DOI:10.1038/nature10750 PubMed ID:22286059

Related PDB Entries:3VGA, 3VG9

Press Release:抗体を用いて創薬標的膜たんぱく質の結晶構造を得ることに成功 －全く新しい阻害機構を持った薬剤の設計が可能に－

109) Haga K, Kruse AC, Asada H, Yurugi-Kobayashi T, Shiroishi M, Zhang C, Weis WI, Okada T, Kobilka BK, Haga T, Kobayashi T.

Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist.

Nature. 2012 Jan 25;482(7386):547-51.

DOI:10.1038/nature10753 PubMed ID:22278061

Related PDB Entries:3UON

Press Release:認知症や心機能の抑制に関係する受容体の立体構造を世界で初めて解明－より効果的で副作用のない治療薬の探索・設計が可能に－

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2011

108) Mizutani K, Yoshioka S, Mizutani Y, Iwata S, Mikami B.

High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins.

Protein Expr Purif. 2011 May;77(1):1-8.

DOI:10.1016/j.pep.2010.12.009 PubMed ID:21172439

107) Ito K, Ito S, Shimamura T, Weyand S, Kawarasaki Y, Misaka T, Abe K, Kobayashi T, Cameron AD, Iwata S.

Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans.

J Mol Biol. 2011 Apr 29;408(2):177-86.

DOI:10.1016/j.jmb.2011.02.028 PubMed ID:21354427

Related PDB Entries:3AIE, 3AIC, 3AIB

Press Release:虫歯の病原因子である酵素の立体構造を世界で初めて解明 ～虫歯の予防物質を探索するための手がかりを得る～

106) Rahman S, Ishizuka-Katsura Y, Arai S, Saijo S, Yamato I, Toyama M, Ohsawa N, Inoue M, Honda K, Terada T, Shirouzu M, Yokoyama S, Iwata S, Murata T.

Expression, purification and characterization of isoforms of peripheral stalk subunits of human V-ATPase.

Protein Expr Purif. 2011 Aug;78(2):181-8.

DOI:10.1016/j.pep.2011.02.012 PubMed ID:21356312

105) de Graaf C, Kooistra AJ, Vischer HF, Katritch V, Kuijer M, Shiroishi M, Iwata S, Shimamura T, Stevens RC, de Esch IJ, Leurs R.

Crystal structure-based virtual screening for fragment-like ligands of the human histamine H(1) receptor.

J Med Chem. 2011 Dec 8;54(23):8195-206.

DOI:10.1021/jm2011589 PubMed ID:22007643

104) Shiroishi M, Kobayashi T, Ogasawara S, Tsujimoto H, Ikeda-Suno C, Iwata S, Shimamura T.

Production of the stable human histamine H1 receptor in Pichia pastoris for structural determination.

Methods. 2011 Dec;55(4):281-6.

DOI:10.1016/j.ymeth.2011.08.015 PubMed ID:21903167

103) Choudhury HG, Cameron AD, Iwata S, Beis K.

Structure and mechanism of the chalcogen-detoxifying protein TehB from Escherichia coli

Biochem J. 2011 Apr 1;435(1):85-91.

DOI:10.1042/BJ20102014 PubMed ID:21244361

Related PDB Entries:2XVM, 2XVA

102) Asada H, Uemura T, Yurugi-Kobayashi T, Shiroishi M, Shimamura T, Tsujimoto H, Ito K, Sugawara T, Nakane T, Nomura N, Murata T, Haga T, Iwata S, Kobayashi T.

Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis.

Microb Cell Fact. 2011 Apr 22;10:24.

DOI:10.1186/1475-2859-10-24 PubMed ID:21513509

101) Tokuda N, Igarashi K, Shimamura T, Yurugi-Kobayashi T, Shiroishi M, Ito K, Sugawara T, Asada H, Murata T, Nomura N, Iwata S, Kobayashi T.

Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium.

Protein Expr Purif. 2011 Sep;79(1):81-7.

DOI:10.1016/j.pep.2011.04.006 PubMed ID:21515379

100) Saijo S, Arai S, Hossain KM, Yamato I, Suzuki K, Kakinuma Y, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Murata T.

Crystal structure of the central axis DF complex of the prokaryotic V-ATPase.

Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19955-60.

DOI:10.1073/pnas.1108810108 PubMed ID:22114184

Related PDB Entries:3AON

Press Release:タンパク質ナノモーターの回転軸の詳細構造を解明－骨粗鬆症に関与する酵素の活性調節領域を同定－

99) Hu NJ, Iwata S, Cameron AD, Drew D.

Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.

Nature. 2011 Oct 5;478(7369):408-11.

DOI:10.1038/nature10450 PubMed ID:21976025

Related PDB Entries:3ZUY, 3ZUX

Press Release:コレステロールを調節するたんぱく質の立体構造を世界で初めて解明－副作用の少ない、高脂血症や動脈硬化症の治療薬の開発へ－

98) Mizutani K, Yamamoto M, Suzuki K, Yamato I, Kakinuma Y, Shirouzu M, Walker JE, Yokoyama S, Iwata S, Murata T.

Structure of the rotor ring modified with N,N'-dicyclohexylcarbodiimide of the Na+-transporting vacuolar ATPase.

Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13474-9.

DOI:10.1073/pnas.1103287108 PubMed ID:21813759

Related PDB Entries:3AOU, 2DB4,

Press Release:V型ATPaseの阻害機構の解明に成功－骨粗鬆症やがんの新薬開発に繋がる成果－

97) Shimamura T, Shiroishi M, Weyand S, Tsujimoto H, Winter G, Katritch V, Abagyan R, Cherezov V, Liu W, Han GW, Kobayashi T, Stevens RC, Iwata S.

Structure of the human histamine H1 receptor complex with doxepin.

Nature. 2011 Jun 22;475(7354):65-70.

DOI:10.1038/nature10236 PubMed ID:21697825

Related PDB Entries:3RZE

Press Release:花粉症・アレルギーの発症因子の立体構造を世界で初めて解明－副作用を抑えた治療薬の探索・設計が可能に－

96) Newstead S, Drew D, Cameron AD, Postis VL, Xia X, Fowler PW, Ingram JC, Carpenter EP, Sansom MS, McPherson MJ, Baldwin SA, Iwata S.

Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2.

EMBO J. 2011 Jan 19;30(2):417-26.

DOI:10.1038/emboj.2010.309 PubMed ID:21131908

Related PDB Entries:2XUT

95) Bill RM, Henderson PJ, Iwata S, Kunji ER, Michel H, Neutze R, Newstead S, Poolman B, Tate CG, Vogel H.

Overcoming barriers to membrane protein structure determination.

Nat Biotechnol. 2011 Apr;29(4):335-40.

DOI:10.1038/nbt.1833 PubMed ID:21478852

94) Ruprecht J, Iwata S, Rothery RA, Weiner JH, Maklashina E, Cecchini G.

Perturbation of the quinone-binding site of complex II alters the electronic properties of the proximal [3Fe-4S] iron-sulfur cluster.

J Biol Chem. 2011 Apr 8;286(14):12756-65.

DOI:10.1074/jbc.M110.209874 PubMed ID:21310949

Related PDB Entries:2WP9

93) Sonoda Y, Newstead S, Hu NJ, Alguel Y, Nji E, Beis K, Yashiro S, Lee C, Leung J, Cameron AD, Byrne B, Iwata S, Drew D.

Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures.

Structure. 2011 Jan 12;19(1):17-25.

DOI:10.1016/j.str.2010.12.001 PubMed ID:21220112

92) Weyand S, Shimamura T, Beckstein O, Sansom MS, Iwata S, Henderson PJ, Cameron AD.

The alternating access mechanism of transport as observed in the sodium-hydantoin transporter Mhp1.

J Synchrotron Radiat. 2011 Jan;18(1):20-3.

DOI:10.1107/S0909049510032449 PubMed ID:21169684

91) Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Misaka T, Iwata S, Kobayashi T, Abe K.

Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein.

Biotechnol Lett. 2011 Jan;33(1):103-7.

DOI:10.1007/s10529-010-0399-1 PubMed ID:20936326

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2010

90) Hino T, Matsumoto Y, Nagano S, Sugimoto H, Fukumori Y, Murata T, Iwata S, Shiro Y.

Structural basis of biological N2O generation by bacterial nitric oxide reductase.

Science. 2010 Dec 17;330(6011):1666-70.

DOI:10.1126/science.1195591 PubMed ID:21109633

Related PDB Entries:3O0R

Press Release:温室効果ガス「亜酸化窒素」を発生させる酵素の立体構造を世界で初めて解明 －嫌気呼吸から酸素呼吸へと呼吸酵素が進化した手がかりを得る－

89) Suzuki N, Hiraki M, Yamada Y, Matsugaki N, Igarashi N, Kato R, Dikic I, Drew D, Iwata S, Wakatsuki S, Kawasaki M.

Crystallization of small proteins assisted by green fluorescent protein.

Acta Crystallogr D Biol Crystallogr. 2010 Oct;66(Pt 10):1059-66.

DOI:10.1107/S0907444910032944 PubMed ID:20944239

Related PDB Entries:3AI5, 3AI4

88) Ito K, Ito S, Shimamura T, Kawarasaki Y, Abe K, Misaka T, Kobayashi T, Iwata S.

Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt 9):1086-8.

DOI:10.1107/S1744309110029714 PubMed ID:20823533

87) Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Masuda K, Ishiguro M, Misaka T, Iwata S, Kobayashi T, Abe K.

Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin.

Biochim Biophys Acta. 2010 Sep;1800(9):986-92.

DOI:10.1016/j.bbagen.2010.06.003 PubMed ID:20542090

86) Weyand S, Iwata S.

Biochemistry. Old gate gets a new look.
(Comment on “Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution. [Science. 2010]”)

Science. 2010 Jul 9;329(5988):151-2.

DOI:10.1126/science.1192680 PubMed ID:20616256

85) Sonoda Y, Cameron A, Newstead S, Omote H, Moriyama Y, Kasahara M, Iwata S, Drew D.

Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins.

FEBS Lett. 2010 Jun 18;584(12):2539-47.

DOI:10.1016/j.febslet.2010.04.015 PubMed ID:20394746

84) Singh S, Hedley D, Kara E, Gras A, Iwata S, Ruprecht J, Strange PG, Byrne B.

A purified C-terminally truncated human adenosine A(2A) receptor construct is functionally stable and degradation resistant.

Protein Expr Purif. 2010 Nov;74(1):80-7.

DOI:10.1016/j.pep.2010.04.018 PubMed ID:20438843

83) Beale J, Lee SY, Iwata S, Beis K.

Structure of the aliphatic sulfonate-binding protein SsuA from Escherichia coli.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Apr 1;66(Pt 4):391-6.

DOI:10.1107/S1744309110006226 PubMed ID:20383006

Related PDB Entries:2X26

82) Shimamura T, Weyand S, Beckstein O, Rutherford NG, Hadden JM, Sharples D, Sansom MS, Iwata S, Henderson PJ, Cameron AD.

Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1.

Science. 2010 Apr 23;328(5977):470-3.

DOI:10.1126/science.1186303 PubMed ID:20413494

Related PDB Entries:2X79

Press Release:細胞膜たんぱく質が物質を細胞内へ運ぶ仕組みを分子レベルで解明 （神経疾患やがんの治療に役立つ可能性）

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2009

81) Arai S, Yamato I, Shiokawa A, Saijo S, Kakinuma Y, Ishizuka-Katsura Y, Toyama M, Terada T, Shirouzu M, Yokoyama S, Iwata S, Murata T.

Reconstitution in vitro of the catalytic portion (NtpA3-B3-D-G complex) of Enterococcus hirae V-type Na+-ATPase.

Biochem Biophys Res Commun. 2009 Dec 18;390(3):698-702.

DOI:10.1016/j.bbrc.2009.10.032 PubMed ID:19833097

80) Maher MJ, Akimoto S, Iwata M, Nagata K, Hori Y, Yoshida M, Yokoyama S, Iwata S, Yokoyama K.

Crystal structure of A3B3 complex of V-ATPase from Thermus thermophilus.

EMBO J. 2009 Dec 2;28(23):3771-9.

DOI:10.1038/emboj.2009.310 PubMed ID:19893485

Related PDB Entries:3GQB

79) Newstead S, Fowler PW, Bilton P, Carpenter EP, Sadler PJ, Campopiano DJ, Sansom MS, Iwata S.

Insights into how nucleotide-binding domains power ABC transport.

Structure. 2009 Sep 9;17(9):1213-22.

DOI:10.1016/j.str.2009.07.009 PubMed ID:19748342

Related PDB Entries:3FVQ

Press Release:

78) Ruprecht J, Yankovskaya V, Maklashina E, Iwata S, Cecchini G.

Structure of Escherichia coli succinate:quinone oxidoreductase with an occupied and empty quinone-binding site.

J Biol Chem. 2009 Oct 23;284(43):29836-46.

DOI:10.1074/jbc.M109.010058 PubMed ID:19710024

Related PDB Entries:2WDR, 2WDV, 2WDQ

77) Sugawara T, Ito K, Shiroishi M, Tokuda N, Asada H, Yurugi-Kobayashi T, Shimamura T, Misaka T, Nomura N, Murata T, Abe K, Iwata S, Kobayashi T.

Fluorescence-based optimization of human bitter taste receptor expression in Saccharomyces cerevisiae.

Biochem Biophys Res Commun. 2009 May 15;382(4):704-10.

DOI:10.1016/j.bbrc.2009.03.089 PubMed ID:19306845

76) Andrell J, Hicks MG, Palmer T, Carpenter EP, Iwata S, Maher MJ.

Crystal structure of the acid-induced arginine decarboxylase from Escherichia coli: reversible decamer assembly controls enzyme activity.

Biochemistry. 2009 May 12;48(18):3915-27.

DOI:10.1021/bi900075d PubMed ID:19298070

Related PDB Entries:2VYC

Press Release:

75) Yurugi-Kobayashi T, Asada H, Shiroishi M, Shimamura T, Funamoto S, Katsuta N, Ito K, Sugawara T, Tokuda N, Tsujimoto H, Murata T, Nomura N, Haga K, Haga T, Iwata S, Kobayashi T.

Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris.

Biochem Biophys Res Commun. 2009 Mar 6;380(2):271-6.

DOI:10.1016/j.bbrc.2009.01.053 PubMed ID:19167344

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2008

74) Shimamura T, Yajima S, Suzuki S, Rutherford NG, O'Reilly J, Henderson PJ, Iwata S.

Crystallization of the hydantoin transporter Mhp1 from Microbacterium liquefaciens.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt 12):1172-4.

DOI:10.1107/S1744309108036920 PubMed ID:19052379

73) Nomura N, Nomura Y, Sussman D, Klein D, Stoddard BL.

Recognition of a common rDNA target site in archaea and eukarya by analogous LAGLIDADG and His-Cys box homing endonucleases.

Nucleic Acids Res. 2008 Dec;36(22):6988-98.

DOI:10.1093/nar/gkn846 PubMed ID:18984620

Related PDB Entries:3E54

72) Drew D, Klepsch MM, Newstead S, Flaig R, De Gier JW, Iwata S, Beis K.

The structure of the efflux pump AcrB in complex with bile acid.

Mol Membr Biol. 2008 Dec;25(8):677-82.

DOI:10.1080/09687680802552257 PubMed ID:19023693

Related PDB Entries:2W1B

71) Newstead S, Hobbs J, Jordan D, Carpenter EP, Iwata S.

Insights into outer membrane protein crystallization.

Mol Membr Biol. 2008 Dec;25(8):631-8.

DOI:10.1080/09687680802526574 PubMed ID:19023694

70) Carpenter EP, Beis K, Cameron AD, Iwata S.

Overcoming the challenges of membrane protein crystallography.

Curr Opin Struct Biol. 2008 Oct;18(5):581-6.

DOI:10.1016/j.sbi.2008.07.001 PubMed ID:18674618

69) Yamamoto M, Unzai S, Saijo S, Ito K, Mizutani K, Suno-Ikeda C, Yabuki-Miyata Y, Terada T, Toyama M, Shirouzu M, Kobayashi T, Kakinuma Y, Yamato I, Yokoyama S, Iwata S, Murata T.

Interaction and stoichiometry of the peripheral stalk subunits NtpE and NtpF and the N-terminal hydrophilic domain of NtpI of Enterococcus hirae V-ATPase.

J Biol Chem. 2008 Jul 11;283(28):19422-31.

DOI:10.1074/jbc.M801772200 PubMed ID:18460472

68) Murata T, Yamato I, Kakinuma Y, Shirouzu M, Walker JE, Yokoyama S, Iwata S.

Ion binding and selectivity of the rotor ring of the Na+-transporting V-ATPase.

Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8607-12.

DOI:10.1073/pnas.0800992105. PubMed ID:18559856

Press Release:イオン輸送性ATPaseの輸送のメカニズムの一端を解明～骨粗鬆症やがん治療の理解に役立つ可能性～

67) Weyand S, Shimamura T, Yajima S, Suzuki S, Mirza O, Krusong K, Carpenter EP, Rutherford NG, Hadden JM, O'Reilly J, Ma P, Saidijam M, Patching SG, Hope RJ, Norbertczak HT, Roach PC, Iwata S, Henderson PJ, Cameron AD.

Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter.

Science. 2008 Oct 31;322(5902):709-13.

DOI:10.1126/science.1164440 PubMed ID:18927357

Related PDB Entries:2JLO, 2JLN

Press Release:細胞膜の「輸送体」が物質を輸送する巧妙な仕組みを解明

66) Jormakka M, Yokoyama K, Yano T, Tamakoshi M, Akimoto S, Shimamura T, Curmi P, Iwata S.

Molecular mechanism of energy conservation in polysulfide respiration.

Nat Struct Mol Biol. 2008 Jul;15(7):730-7.

DOI:10.1038/nsmb.1434 PubMed ID:18536726

Related PDB Entries:2VPZ, 2VPY, 2VPX, 2VPW

65) Ito K, Sugawara T, Shiroishi M, Tokuda N, Kurokawa A, Misaka T, Makyio H, Yurugi-Kobayashi T, Shimamura T, Nomura N, Murata T, Abe K, Iwata S, Kobayashi T.

Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae.

Biochem Biophys Res Commun. 2008 Jul 11;371(4):841-5.

DOI:10.1016/j.bbrc.2008.04.182 PubMed ID:18474222

64) Kouwen TR, Andrell J, Schrijver R, Dubois JY, Maher MJ, Iwata S, Carpenter EP, van Dijl JM.

Thioredoxin A active-site mutants form mixed disulfide dimers that resemble enzyme-substrate reaction intermediates.

J Mol Biol. 2008 Jun 6;379(3):520-34.

DOI:10.1016/j.jmb.2008.03.077 PubMed ID:18455736

Related PDB Entries:2VOC

63) Drew D, Newstead S, Sonoda Y, Kim H, von Heijne G, Iwata S.

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.

Nat Protoc. 2008;3(5):784-98.

DOI:10.1038/nprot.2008.44 PubMed ID:18451787

62) Newstead S, Ferrandon S, Iwata S.

Rationalizing alpha-helical membrane protein crystallization.

Protein Sci. 2008 Mar;17(3):466-72.

DOI:10.1110/ps.073263108 PubMed ID:18218713

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2007

61) Tanabe M, Mirza O, Bertrand T, Atkins HS, Titball RW, Iwata S, Brown KA, Byrne B.

Structures of OppA and PstS from Yersinia pestis indicate variability of interactions with transmembrane domains.

Acta Crystallogr D Biol Crystallogr. 2007 Nov;63(Pt 11):1185-93.

PubMed ID:18007034

Related PDB Entries:2Z23, 2Z22

60) Guan L, Mirza O, Verner G, Iwata S, Kaback HR.

Structural determination of wild-type lactose permease.

Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15294-8.

PubMed ID:17881559

Related PDB Entries:2V8N

59) Newstead S, Kim H, von Heijne G, Iwata S, Drew D.

High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.

Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13936-41.

PubMed ID:17709746

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2006

58) Horsefield R, Yankovskaya V, Sexton G, Whittingham W, Shiomi K, Omura S, Byrne B, Cecchini G, Iwata S.

Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction.

J Biol Chem. 2006 Mar 17;281(11):7309-16.

PubMed ID:16407191

Related PDB Entries:2ACZ

57) Mirza O, Guan L, Verner G, Iwata S, Kaback HR.

Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY.

EMBO J. 2006 Mar 22;25(6):1177-83. Erratum in EMBO J. 2006 May 3;25(9):2038.

PubMed ID:16525509

Related PDB Entries:2CFQ, 2CFP

56) Shimizu-Ibuka A, Morita Y, Terada T, Asakura T, Nakajima K, Iwata S, Misaka T, Sorimachi H, Arai S, Abe K.

Crystal structure of neoculin: insights into its sweetness and taste-modifying activity.

J Mol Biol. 2006 May 26;359(1):148-58.

PubMed ID:16616933

Related PDB Entries:2D04

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2005

55) Makyio H, Iino R, Ikeda C, Imamura H, Tamakoshi M, Iwata M, Stock D, Bernal RA, Carpenter EP, Yoshida M, Yokoyama K, Iwata S.

Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus.

EMBO J. 2005 Nov 16;24(22):3974-83.

PubMed ID:16281059

Related PDB Entries:2D00

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2004

54) Abramson J, Iwata S, Kaback HR.

Lactose permease as a paradigm for membrane transport proteins (Review).

Mol Membr Biol. 2004 Jul-Aug;21(4):227-36.

PubMed ID:15371012

53) Iwata S, Barber J.

Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily.

Curr Opin Struct Biol. 2004 Aug;14(4):413-9.

PubMed ID:15313234

52) Shimamura T, Shamotienko O, Akhtar S, Dolly JO, Iwata S.

Crystallization and preliminary X-ray analysis of pseudo-merohedrally twinned crystals of the full-length beta(2) subunit of the Kv1 K(+) channel from Rattus norvegicus.

Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):912-4.

PubMed ID15103137:

51) Horsefield R, Iwata S, Byrne B.

Complex II from a structural perspective.

Curr Protein Pept Sci. 2004 Apr;5(2):107-18.

PubMed ID:15078221

50) Shimamura T, Koike-Takeshita A, Yokoyama K, Masui R, Murai N, Yoshida M, Taguchi H, Iwata S.

Crystal structure of the native chaperonin complex from Thermus thermophilus revealed unexpected asymmetry at the cis-cavity.

Structure. 2004 Aug;12(8):1471-80.

PubMed ID:15296740

Related PDB Entries:1WE3

49) Ferreira KN, Iverson TM, Maghlaoui K, Barber J, Iwata S.

Architecture of the photosynthetic oxygen-evolving center.

Science. 2004 Mar 19;303(5665):1831-8.

PubMed ID:14764885

Related PDB Entries:1S5L

48) Jormakka M, Richardson D, Byrne B, Iwata S.

Architecture of NarGH reveals a structural classification of Mo-bisMGD enzymes.

Structure. 2004 Jan;12(1):95-104.

PubMed ID:14725769

Related PDB Entries:1R27

47) Iwata M, Imamura H, Stambouli E, Ikeda C, Tamakoshi M, Nagata K, Makyio H, Hankamer B, Barber J, Yoshida M, Yokoyama K, Iwata S.

Crystal structure of a central stalk subunit C and reversible association/dissociation of vacuole-type ATPase.

Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):59-64.

PubMed ID:14684831

Related PDB Entries:1R5Z

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2003

46) Abramson J, Smirnova I, Kasho V, Verner G, Iwata S, Kaback HR.

The lactose permease of Escherichia coli: overall structure, the sugar-binding site and the alternating access model for transport.

FEBS Lett. 2003 Nov 27;555(1):96-101.

PubMed ID:14630326

45) Shimamura T, Koike-Takeshita A, Yokoyama K, Yoshida M, Taguchi H, Iwata S.

Crystallization of the chaperonin GroEL-GroES complex from Thermus thermophilus HB8.

Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1632-4.

PubMed ID:12925795

44) Jormakka M, Byrne B, Iwata S.

Formate dehydrogenase--a versatile enzyme in changing environments.

Curr Opin Struct Biol. 2003 Aug;13(4):418-23.

PubMed ID:12948771

43) Jormakka M, Byrne B, Iwata S.

Protonmotive force generation by a redox loop mechanism.

FEBS Lett. 2003 Jun 12;545(1):25-30.

PubMed ID:12788488

42) Cecchini G, Maklashina E, Yankovskaya V, Iverson TM, Iwata S.

Variation in proton donor/acceptor pathways in succinate:quinone oxidoreductases.

FEBS Lett. 2003 Jun 12;545(1):31-8.

PubMed ID:12788489

41) Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S.

Structure and mechanism of the lactose permease of Escherichia coli.

Science. 2003 Aug 1;301(5633):610-5.

PubMed ID:12893935

Related PDB Entries:1PV7, 1PV6

Press Release:

40) Horsefield R, Yankovskaya V, Tornroth S, Luna-Chavez C, Stambouli E, Barber J, Byrne B, Cecchini G, Iwata S.

Using rational screening and electron microscopy to optimize the crystallization of succinate:ubiquinone oxidoreductase from Escherichia coli.

Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):600-2.

PubMed ID:12595738

39) Yankovskaya V, Horsefield R, Tornroth S, Luna-Chavez C, Miyoshi H, Leger C, Byrne B, Cecchini G, Iwata S.

Architecture of succinate dehydrogenase and reactive oxygen species generation.

Science. 2003 Jan 31;299(5607):700-4.

PubMed ID:12560550

Related PDB Entries:1NEN, 1NEK

Press Release:

38) Iwata M, Abramson J, Byrne B, Iwata S.

Structure and function of quinone binding membrane proteins.

Adv Protein Chem. 2003;63:151-76.

PubMed ID:12629970

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2002

37) Svensson-Ek M, Abramson J, Larsson G, Tornroth S, Brzezinski P, Iwata S.

The X-ray crystal structures of wild-type and EQ(I-286) mutant cytochrome c oxidases from Rhodobacter sphaeroides.

J Mol Biol. 2002 Aug 9;321(2):329-39.

PubMed ID:12144789

Related PDB Entries:1M57, 1M6

36) Jormakka M, Tornroth S, Byrne B, Iwata S.

Molecular basis of proton motive force generation: structure of formate dehydrogenase-N.

Science. 2002 Mar 8;295(5561):1863-8.

PubMed ID:11884747

Related PDB Entries:1KQG, 1KQF

35) Tornroth S, Yankovskaya V, Cecchini G, Iwata S.

Purification, crystallisation and preliminary crystallographic studies of succinate:ubiquinone oxidoreductase from Escherichia coli.

Biochim Biophys Acta. 2002 Jan 17;1553(1-2):171-6.

PubMed ID:11803025

34) Byrne B, Iwata S.

Membrane protein complexes.

Curr Opin Struct Biol. 2002 Apr;12(2):239-43.

PubMed ID:11959503

33) Jormakka M, Tornroth S, Abramson J, Byrne B, Iwata S.

Purification and crystallization of the respiratory complex formate dehydrogenase-N from Escherichia coli.

Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):160-2.

PubMed ID:11752799

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2001

32) Warkentin E, Mamat B, Sordel-Klippert M, Wicke M, Thauer RK, Iwata M, Iwata S, Ermler U, Shima S.

Structures of F420H2:NADP+ oxidoreductase with and without its substrates bound.

EMBO J. 2001 Dec 3;20(23):6561-9.

PubMed ID:11726492

Related PDB Entries:1JAY, 1JAX

31) Abramson J, Svensson-Ek M, Byrne B, Iwata S.

Structure of cytochrome c oxidase: a comparison of the bacterial and mitochondrial enzymes.

Biochim Biophys Acta. 2001 Jan 12;1544(1-2):1-9.

PubMed ID:11341911

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2000

30) Abramson J, Larsson G, Byrne B, Puustinen A, Garcia-Horsman A, Iwata S.

Purification, crystallization and preliminary crystallographic studies of an integral membrane protein, cytochrome bo3 ubiquinol oxidase from Escherichia coli.

Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):1076-8.

PubMed ID:10944359

29) Andreu AL, Checcarelli N, Iwata S, Shanske S, DiMauro S.

A missense mutation in the mitochondrial cytochrome b gene in a revisited case with histiocytoid cardiomyopathy.

Pediatr Res. 2000 Sep;48(3):311-4.

PubMed ID:10960495

28) Byrne B, Abramson J, Jansson M, Holmgren E, Iwata S.

Fusion protein approach to improve the crystal quality of cytochrome bo(3) ubiquinol oxidase from Escherichia coli.

Biochim Biophys Acta. 2000 Aug 15;1459(2-3):449-55.

PubMed ID:11004462

27) Abramson J, Riistama S, Larsson G, Jasaitis A, Svensson-Ek M, Laakkonen L, Puustinen A, Iwata S, Wikstrom M.

The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site.

Nat Struct Biol. 2000 Oct;7(10):910-7.

PubMed ID:11017202

Related PDB Entries:1FFT

26) Sivars U, Abramson J, Iwata S, Tjerneld F.

Affinity partitioning of a poly(histidine)-tagged integral membrane protein, cytochrome bo3 ubiquinol oxidase, in a detergent--polymer aqueous two-phase system containing metal-chelating polymer.

J Chromatogr B Biomed Sci Appl.2000 Jun 23;743(1-2):307-16.

PubMed ID:10942301

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1999

25) Andreu AL, Hanna MG, Reichmann H, Bruno C, Penn AS, Tanji K, Pallotti F, Iwata S, Bonilla E, Lach B, Morgan-Hughes J, DiMauro S.

Exercise intolerance due to mutations in the cytochrome b gene of mitochondrial DNA.

N Engl J Med. 1999 Sep 30;341(14):1037-44.

PubMed ID:10502593

24) Iwata M, Bjorkman J, Iwata S.

Conformational change of the Rieske [2Fe-2S] protein in cytochrome bc1 complex.

J Bioenerg Biomembr. 1999 Jun;31(3):169-75.

PubMed ID:10591523

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1998

23) Iwata S, Lee JW, Okada K, Lee JK, Iwata M, Rasmussen B, Link TA, Ramaswamy S, Jap BK.

Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex.

Science. 1998 Jul 3;281(5373):64-71.

PubMed ID:9651245

Related PDB Entries:1BE3, 1BGY

22) Iwata S.

Structure and function of bacterial cytochrome c oxidase.

J Biochem. 1998 Mar;123(3):369-75.

PubMed ID:9538216

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1996

21) Fushinobu S, Kamata K, Iwata S, Sakai H, Ohta T, Matsuzawa H.

Allosteric activation of L-lactate dehydrogenase analyzed by hybrid enzymes with effector-sensitive and -insensitive subunits.

J Biol Chem. 1996 Oct 11;271(41):25611-6.

PubMed ID:8810336

20) Ostermeier C, Iwata S, Michel H.

Cytochrome c oxidase.

Curr Opin Struct Biol. 1996 Aug;6(4):460-6.

PubMed ID:8794157

19) Link TA, Iwata S.

Functional implications of the structure of the 'Rieske' iron-sulfur protein of bovine heart mitochondrial cytochrome bc1 complex.

Biochim Biophys Acta. 1996 Jul 18;1275(1-2):54-60.

PubMed ID:8688452

18) Riistama S, Puustinen A, Garcia-Horsman A, Iwata S, Michel H, Wikstrom M.

Channelling of dioxygen into the respiratory enzyme.

Biochim Biophys Acta. 1996 Jul 18;1275(1-2):1-4.

PubMed ID:8688439

17) Iwata S, Saynovits M, Link TA, Michel H.

Structure of a water soluble fragment of the 'Rieske' iron-sulfur protein of the bovine heart mitochondrial cytochrome bc1 complex determined by MAD phasing at 1.5 A resolution.

Structure. 1996 May 15;4(5):567-79.

PubMed ID:8736555

Related PDB Entries:1RIE

16) Link TA, Saynovits M, Assmann C, Iwata S, Ohnishi T, von Jagow G.

Isolation, characterisation and crystallisation of a water-soluble fragment of the Rieske iron-sulfur protein of bovine heart mitochondrial bc1 complex.

Eur J Biochem. 1996 Apr 1;237(1):71-5.

PubMed ID:8620896

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1995

15) Dohse B, Mathis P, Wachtveitl J, Laussermair E, Iwata S, Michel H, Oesterhelt D.

Electron transfer from the tetraheme cytochrome to the special pair in the Rhodopseudomonas viridis reaction center: effect of mutations of tyrosine L162.

Biochemistry. 1995 Sep 12;34(36):11335-43.

PubMed ID:7547861

14) Ostermeier C, Iwata S, Ludwig B, Michel H.

Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase.

Nat Struct Biol. 1995 Oct;2(10):842-6.

PubMed ID:7552705

Related PDB Entries:1MQK

13) Iwata S, Ostermeier C, Ludwig B, Michel H.

Structure at 2.8 A resolution of cytochrome c oxidase from Paracoccus denitrificans.

Nature. 1995 Aug 24;376(6542):660-9.

PubMed ID: 7651515

Related PDB Entries:1QLE

12) Kleymann G, Iwata S, Wiesmuller KH, Ludwig B, Haase W, Michel H.

Immunoelectron microscopy and epitope mapping with monoclonal antibodies suggest the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol:cytochrome-c oxidoreductases.x

Eur J Biochem. Wiesmuller KH, Ludwig B, Haase W, Michel H.

PubMed ID:7601123

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1994

11) Iwata S, Kamata K, Yoshida S, Minowa T, Ohta T.

T and R states in the crystals of bacterial L-lactate dehydrogenase reveal the mechanism for allosteric control.

Nat Struct Biol. 1994 Mar;1(3):176-85.

PubMed ID:7656036

Related PDB Entries:1LTH

10) Iwata S, Yoshida S, Ohta T.

A regular 1:1 complex of two allosteric states in the single crystal of L-lactate dehydrogenase from Bifidobacterium longum.

J Mol Biol. 1994 Feb 25;236(3):958-9.

PubMed ID:8114106

Related PDB Entries:1LTH

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1993

9) Tanokura M, Sasaki H, Muramatsu T, Iwata S, Hamaya T, Takizawa T, Takahashi K.

A new crystal form of proteinase A, a non-pepsin-type acid proteinase from Aspergillus niger var. macrosporus.

J Biochem. 1993 Oct;114(4):457-8.

PubMed ID:8276753

8) Iwata S, Ohta T.

Molecular basis of allosteric activation of bacterial L-lactate dehydrogenase.

J Mol Biol. 1993 Mar 5;230(1):21-7.

PubMed ID:

Related PDB Entries:1LLD

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1992

7) Tanokura, M., Matsuzaki, H., Iwata, S., Nakagawa, A., Hamaya, T., Takizawa, T., and Takahashi, K.

Effect of dimethylsulfoxide on the crystallization of Aspergillus niger proteinase A.

Proc. Japan Acad. Sci. 1992; 67B:209-212

6) Tanokura M, Matsuzaki H, Iwata S, Nakagawa A, Hamaya T, Takizawa T, Takahashi K.

Crystallization and preliminary X-ray investigation of proteinase A, a non-pepsin-type acid proteinase from Aspergillus niger var. macrosporus.

J Mol Biol. 1992 Jan 5;223(1):373-5.

PubMed ID:1731082

5) Ohta T, Yokota K, Minowa T, Iwata S.

Mechanism of allosteric transition of bacterial L-lactate dehydrogenase.

Faraday Discuss. 1992;(93):153-62.

PubMed ID:1290930

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1991

4) Koide S, Iwata S, Matsuzawa H, Ohta T.

Crystallization of allosteric L-lactate dehydrogenase from Thermus caldophilus and preliminary crystallographic data.

J Biochem. 1991 Jan;109(1):6-7.

PubMed ID:2016275

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1989

3) Iwata S, Minowa T, Mikami B, Morita Y, Ohta T.

Crystallization of and preliminary crystallographic data for allosteric L-lactate dehydrogenase from Bifidobacterium longum.

J Biochem. 1989 Oct;106(4):558-9.

PubMed ID:2691505

2) Minowa T, Iwata S, Sakai H, Masaki H, Ohta T.

Sequence and characteristics of the Bifidobacterium longum gene encoding L-lactate dehydrogenase and the primary structure of the enzyme: a new feature of the allosteric site.

Gene. 1989 Dec 21;85(1):161-8.

PubMed ID:2695396

1) Iwata, S., Minowa, T., Sakai, H. and Ohta, T.

Amino acid residues in the allosteric site of L-lactate dehydrogenase from Bifidobacterium longum.

Agril. and Bio. Chem. 1989; 53: 3365-3366

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